Lei Zhao, Yujia Chen, Kuan Wu, He Yan, Xingan Hao, Yunfeng Wu. Application of Fatty Acids as Antiviral Agents Against Tobacco Mosaic Virus

Application of Fatty Acids as Antiviral Agents Against Tobacco Mosaic Virus

Lei Zhao, Yujia Chen, Kuan Wu, He Yan, Xingan Hao, Yunfeng Wu.

Pesticide Biochemistry and Physiology

DOI:10.1016/j.pestbp.2017.05.005

Abstract：Numer ous studies reported fattyacids (FAs) affectingbasalresistancetobacterialandfungalpathogensinplants,but limited reports focused on antiviral agents. In this study, oleic acid was separated from cottonseed oil sludge byantiviral bioassay-guidedmethods. Antiviral activity of FAs wascompared with that ofNingnanmycin. Subse-quently, effects of FAs on defense-related enzymes (PAL, POD) and defense-related genes (PR-1a, PR-5) were studied. FAs presented moderate antiviral activity, which is close to that of Ningnanmycin, and activities of PAL and POD were higher in oleic-acid-treated tobacco leaves than those inoculated with tobacco mosaic virus (TMV) and water-treated tobacco. In oleic-acid-treated tobacco, expression levels of PR-1a and PR-5 genes rap idlyincreasedfrom days 1–3.All results showedthat FAs canincreaseresistanceagainst TMV,and relatedmechanism can be attributed to activated expressions of a number of defense-related genes, suggesting that FAs can potentially act as pesticides for integrated control of plant viruses in the future.