Genome Sequence Resource for Erysiphe necator NAFU1, a Grapevine Powdery Mildew Isolate Identified in Shaanxi Province of China
Xingyuan Zhang, Bo Mu, Kaicheng Cui, Min Liu, Guihua Ke, Yongtao Han, Ying Wu, Shunyuan Xiao, Yingqiang Wen
Molecular Plant-microbe Interactions
DOI: 10.1094/mpmi-03-21-0061-a
Abstract
Erysiphe necator is an economically important biotrophic fungal pathogen responsible for powdery mildew disease on grapevine. Currently, genome sequences are available for only a few Erysiphe necator isolates from USA. Based on the combination of Nanopore and Illumina sequencing technologies, we present here the complete genome assembly for an isolate of E. necator NAFU1 identified in China. We acquired a total of 15.93 Gb raw reads. These reads were processed into a 61.12 Mb genome assembly containing 73 contigs with the N50 of 2.06 Mb and a maximum length of 6.05 Mb. Combining the results of three gene-prediction modules, i.e. an evidence-based gene modeler (EVidenceModeler or EVM), an ab initio gene modeler, and a homology-based gene modeler, we predicted 7235 protein-coding genes in the assembled genome of E. necator NAFU1. This information will facilitate studies of genome evolution and pathogenicity mechanisms of E. necator and other powdery mildew species through comparative genome sequence analysis and other molecular genetic tools.