Pingchuan Deng, Meng Wang, Kewei Feng, Licao Cui, Wei Tong, Weining Song, Xiaojun Nie. Genome-Wide Characterization of Microsatellites in Triticeae Species: Abundance, Distribution and Evolution

Genome-Wide Characterization of Microsatellites in Triticeae Species: Abundance, Distribution and Evolution.

Pingchuan Deng, Meng Wang, Kewei Feng, Licao Cui, Wei Tong, Weining Song, Xiaojun Nie. 

Scientific RepoRts

DOI: 10.1038/srep32224

Abstract: Microsatellites are an important constituent of plant genome and distributed across entire genome. In this study, genome-wide analysis of microsatellites in 8 Triticeae species and 9 model plants revealed that microsatellite characteristics were similar among the Triticeae species. Furthermore, genome-wide microsatellite markers were designed in wheat and then used to analyze the evolutionary relationship of wheat and other Triticeae species. Results displayed that Aegilops tauschii was found to be the closest species to Triticum aestivum , followed by Triticum urartu , Triticum turgidum and Aegilops speltoides , while Triticum monococcum , Aegilops sharonensis and Hordeum vulgare showed a relatively lower PCR amplifcation effectivity. Additionally, a signifcantly higher PCR amplifcation effectivity was found in chromosomes at the same subgenome than its homoeologous when these markers were subjected to search against different chromosomes in wheat. After a rigorous screening process, a total of 20,666 markers showed high amplifcation and polymorphic potential in wheat and its relatives, which were integrated with the public available wheat markers and then anchored to the genome of wheat (CS). This study not only provided the useful resource for SSR markers development in Triticeae species, but also shed light on the evolution of polyploid wheat from the perspective of microsatellites.