Yanqing Wang, Shuxiu Li, Xutong Zhang, Yuejin Wang, Chaohong Zhang. Isolation and Analysis of Differentially Expressed Genes During Ovule Abortion in the Seedless Grape

Isolation and Analysis of Differentially Expressed Genes During Ovule Abortion in the Seedless Grape.

Yanqing Wang, Shuxiu Li, Xutong Zhang, Yuejin Wang, Chaohong Zhang.

Scientia Horticulturae

DOI:10.1016/j.scienta.2016.09.014

Abstract: The propagation of seedless grapes is currently one of the most important global trends in agricultural production and consumption. However, stenospermocarpy has not been characterized at the molecular level to date. In the present study, cDNA sequence-related amplified polymorphism (SRAP) analysis using 17 SRAP primers was utilized to identify differentially expressed genes during ovule development in Vitis vinifera L. cv. Youngle and V. Vinifera L. cv. Pinot noir. A total of 24 ESTs were detected, with lengths ranging from 147 bp to 1785 bp. These ESTs were deposited to GenBank (Accession numbers JK818295–JK818319). EST differential expression analysis was performed in two grape species, followed by cloning, from which 8 genes were selected for expression profiling in different tissues during ovule development of V. vinifera L.cv. Youngle and V. Vinifera L. cv. Pinot noir. The genes YG7931, YG38221, and YG5571 showed stage-specific expression during ovule development.