Can Chen, Junfeng Pan, Xiaobing Yang, Chenghao Guo, Wei Ding, Meiru Si, Yi Zhang, Xihui Shen, Yao Wang. Global Transcriptomic Analysis of the Response of Corynebacterium Glutamicum to Vanillin

Global Transcriptomic Analysis of the Response of Corynebacterium Glutamicum to Vanillin.

Can Chen, Junfeng Pan, Xiaobing Yang, Chenghao Guo, Wei Ding, Meiru Si, Yi Zhang, Xihui Shen, Yao Wang. 

PLoS ONE  

DOI:10.1371/journal.pone.0164955

Abstract: Lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. Vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. To assess the response of Corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. The transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related to the stress response, ribosome/translation, protein secretion, and the cell envelope. Moreover, deletion of the sigH or msrA gene in C. glutamicum resulted in a decrease in cell viability under vanillin stress. These insights will promote further engineering of model industrial strains, with enhanced tolerance or degradation ability to vanillin to enable suitable production of biofuels and bio-based chemicals from lignocellulosic biomass.