Yanlin Li, Dandan Wang, Congcong Sun, Xiaochen Hu, Xiaoqian Mu, Jingjiang Hu, Yongqing Yang, Yanfeng Zhang, Chang Gen Xie, Xiaona Zhou. Molecular Characterization of an AtPYL1-like Protein, BrPYL1, as Aputative ABA Receptor in Brassica Rapa

Molecular Characterization of an AtPYL1-like Protein, BrPYL1, as Aputative ABA Receptor in Brassica Rapa.

Yanlin Li, Dandan Wang, Congcong Sun, Xiaochen Hu, Xiaoqian Mu, Jingjiang Hu, Yongqing Yang, Yanfeng Zhang, Chang Gen Xie, Xiaona Zhou.

Biochemical and Biophysical Research Communications

DOI:10.1016/j.bbrc.2017.04.116

Abstract: Abscisic acid (ABA)-induced physiological changes are conserved in many land plants and underlie their responses to environmental stress and pathogens. The PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULA-TORY COMPONENTS OF ABA RECEPTORS (PYLs)-type receptors perceive the ABA signal and initiate signal transduction. Here, we show that the genome of Brassica rapa encodes 24 putative AtPYL-like proteins. The AtPYL-like proteins in Brassica rapa (BrPYLs) can also be classified into 3 subclasses. We found that nearly all BrPYLs displayed high expression in at least one tissue. Overexpression of BrPYL1 conferred ABA hypersensitivity to Arabidopsis. Further, ABA activated the expression of an ABA-responsive reporter in Arabidopsis protoplasts expressing BrPYL1. Overall, these results suggest that BrPYL1 is a putative functional ABA receptor in Brassica rapa.